책임운영기관 농촌진흥청 국립축산과학원 (로고)

This study was conducted to identify Chikso and other breeds through the evaluation of molecular biological characteristics instead of the existing method of distinguishing the Chikso breed through difficult-to-define hair color. We developed single nucleotide polymorphism (SNP) markers that discriminate between Chikso and non-Chikso (Hanwoo, Holstein, Jeju Black, and White Hanwoo) using the Illumina BovineHD SNP 777K Bead chip and 5 other breeds of cattle. A total of 112 SNPs with a minor allele frequency (MAF) difference of 0.3 or more were obtained between Chikso and other breeds. A linear model incorporating genetic variation was created using stepwise regression. The 21 SNP markers for the Chikso discrimination were finally constructed by substituting them into the additive model. The genetic marker set developed showed 100% accuracy for 96 Chikso and 190 non-Chikso breeds. Using these markers, the Chikso breed can therefore be accurately identified through molecular biological methods. It will be possible to use these methods in various fields of agriculture and food production through the systematic and scientific establishment of livestock genetic resources. The results of this study constitute a unique biological resource in Korea based on genetic research on the Chikso breed and could be used as basic data to enhance its value as a breeder.