책임운영기관 농촌진흥청 국립축산과학원 (로고)

This study endeavored to develop a vitrification device and examine the survival rates of pig oocytes following vitrification and warming. General lab equipment, such as nylon mesh and inoculation loop, were used to develop the vitrification device. The survival rate was examined using in vitro matured pig oocytes. Our results reveal that 40 and 50 μm nylon mesh showed significantly (P<0.05) higher survival rates (71.7 and 75.5%, respectively) compared to other mesh sizes of 30, 60, and 70 μm (58.3, 65.0, and 56.7%, respectively), and were comparable to survival obtained with Cryotop (71.7%). Cryoinjuries by dislocation of oocytes (with 60 and 70 μm mesh) and higher volume of vitrification solution (30 μm) resulted in reduced survival rates. However, the oocytes could be safely located and excessive vitrification solution could be removed easily in the 40 and 50 μm groups. In conclusion, we confirm that 40 and 50 μm of nylon mesh is very useful for preserving a high amount of pig oocytes with survival rates comparable to a commercialized vitrification device.