책임운영기관 농촌진흥청 국립축산과학원 (로고)

Background Lawsonia intracellularis is the causative agent of proliferative enteropathy and is associated with several outbreaks, causing substantial economic loss to the porcine industry. Objectives In this study, we focused on demonstrating the protective effect in the mouse model through the immunological bases of two vaccine strains against porcine proliferative enteritis. Methods We used live-attenuated Salmonella Typhimurium secreting two selected immunogenic L. intracellularis antigens (Lawsonia autotransporter A epitopes and flagellin–peptidoglycan-associated lipoprotein–flagellin) as the vaccine carrier. The constructs were cloned into a Salmonella expression vector (pJHL65) and transformed into the S. Typhimurium strain (JOL912). The expression of immunogenic proteins within Salmonella was evaluated via immunoblotting. Results Immunizing BALB/c mice orally and subcutaneously induced high levels of L. intracellularis-specific systemic immunoglobulin G and mucosal secretory immunoglobulin A. In immunized mice, there was significant upregulation of interferon-γ and interleukin-4 cytokine mRNA and an increase in the subpopulations of cluster of differentiation 4+ and cluster of differentiation 8+ T lymphocytes upon splenocytes re-stimulation with L. intracellularis antigens. We observed significant protection in C57BL/6 mice against challenge with 106.9 times the median tissue culture infectious dose of L. intracellularis or 2×109 CFU of the virulent S. Typhimurium strain. Immunizing mice with either individual vaccine strains or co-mixture inhibited bacterial proliferation, with a marked reduction in the percentage of mice shedding Lawsonia in their feces. Conclusions Salmonella-mediated L. intracellularis gene delivery induces robust humoral and cellular immune reactions, leading to significant protection against L. intracellularis and salmonellosis