책임운영기관 농촌진흥청 국립축산과학원 (로고)

<P>Abstract<P>Hydrogen is an alternative fuel for transportation vehicles because it is clean, sustainable, and highly flammable. However, the production of hydrogen from lignocellulosic biomass by microorganisms presents challenges. This microbial process involves multiple complex steps, including thermal, chemical, and mechanical treatment of biomass to remove hemicellulose and lignin, as well as enzymatic hydrolysis to solubilize the plant cell walls. These steps not only incur costs but also result in the production of toxic hydrolysates, which inhibit microbial growth. A hyper-thermophilic bacterium of <I>Caldicellulosiruptor bescii</I> can produce hydrogen by decomposing and fermenting plant biomass without the need for conventional pretreatment. It is considered as a consolidated bioprocessing (CBP) microorganism. This review summarizes the basic scientific knowledge and hydrogen-producing capacity of <I>C. bescii</I>. Its genetic system and metabolic engineering strategies to improve hydrogen production are also discussed.</P></P><P>Key points<P><I>&#x2022; Hydrogen is an alternative and eco-friendly fuel.</I></P><P><I>&#x2022; Caldicellulosiruptor bescii produces hydrogen with a high yield in nature.</I></P><P><I>&#x2022; Metabolic engineering can make C. bescii to improve hydrogen production.</I></P></P>